Harley Quinn

Kaley Cuoco

Harley Quinn (voice)

Lake Bell

Poison Ivy (voice)

Ron Funches

King Shark (voice)

JB Smoove

Frank the Plant (voice) / Baby Frank the Plant (voice)

Tony Hale

Dr. Psycho (voice) / Dr. Psycho / Man / Hench (voice) / Dr. Psycho / Snow Rat (voice) / Dr. Psycho / Felix Faust (voice) / Dr Psycho / Steve (voice) / Dr. Psycho / Businessman (voice) / Dr. Psycho / Man / Lead Goon (voice)

Jason Alexander

Sy Borgman (voice)

Diedrich Bader

Batman (voice) / Bruce Wayne (voice) / Batman / Loving Guard (voice) / Trainer (voice) / Batman / Thomas Wayne (voice) / Bruce Wayne / Sven (voice) / Gorilla Grodd (voice) / Bruce Wayne / Surgeon / Goon 2 (voice) / Batman / No Pinkie Pete (voice) / Batman / Goon (voice) / Batman / Gorilla Grodd / Doctor (voice)

Christopher Meloni

Commissioner Gordon (voice) / Jim Gordon / Jeffrey the Parrot (voice) / Jim Gordon (voice) / Commissioner Gordon / Guard (voice) / Jim Gordon / Mr. Veronica Cale / Jeffey the Parrot (voice) / Commissioner Gordon / Two-Face Goon / Bouncer (voice)

Alan Tudyk

Clayface / The Joker (voice) / Clayface (voice) / The Joker (voice) / Clayface / Perry White (voice) / Clayface / Condiment King (voice) / The Joker / Clayface (voice) / Clayface / Two-Face Goon / Manager (voice) / Clayface / Firefly (voice) / The Joker / Clayface / Fancy Guard (voice) / Clayface / Singing Fish (voice) / The Joker / Dr. Trap (voice) / The Joker / Calendar man (voice) / Clayface / Doctor Trap / Cool Guard (voice) / Clayface / Clayface #2 (voice) / The Joker / Clayface / Hot Southern Bartender (voice) / Clayface / Two-Face Goon / Student (voice) / The Joker / Firefly (voice)

Giancarlo Esposito

Lex Luthor (voice)

James Adomian

Bane/Vincent Edge/Animal Control Officer (voice) / Bane (voice) / Bane / Clock King (voice) / Snowflame / Chef Kanue / Pool Boy #1 (voice) / Bane / Owner (voice) / Bane / Talkative Teddy Tilapia (voice) / Bane / Sad Dad (voice) / Bane / Terrorist #1 / CEO (voice) / Bane / RIddler Goon / Penguin Goon (voice) / Bane / Ian (voice) / Bane / Ratcatcher (voice) / Bane / Nelvin (voice) / Chaz (voice) / Bane / Jeffrey's Handler (voice) / Bane / Snowflame / Legion of Doom Security Guard (voice)

Matt Oberg

Kiteman (voice) / Killer Croc (voice) / Kiteman / KGBeast (voice) / Kiteman / Bane Goon (voice) / Kite Man (voice) / Killer Croc / Banker (voice) / Kiteman / Uncool Guard (voice) / Killer Croc / Thurston / Waiter (voice)